© Copyright 2008 by the Society for Neuro-Oncology
Received October 17, 2006
Accepted July 18, 2007
Basic and Translational Investigations
|
Identification of a novel proliferation-related protein, WHSC1 4a, in human
gliomas
Jie Li 1,
Chunyue Yin 1,
Hiroaki Okamoto 1,
Brian M. Balgley 2,
Cheng S. Lee 2,
Kristy Yuan 1,
Barbara Ikejiri 1,
Sven Glasker 1,
Alexander O. Vortmeyer 1,
Edward H. Oldfield 1,
Robert J. Weil 3,
Zhengping Zhuang 1*
1 Surgical Neurology Branch, National Institute of Neurological Disorders and
Stroke, National Institutes of Health, Bethesda, MD, USA
2 Calibrant Biosystems, Gaithersburg, MD, USA
3 Brain Tumor and Neuro-Oncology Center, Department of Neurosurgery, Cleveland
Clinic, Cleveland, OH, USA
* To whom correspondence should be addressed. E-mail: zhuangp{at}ninds.nih.gov.
 |
Abstract |
|---|
Dynamic changes in the expression of multiple genes appear to be common features that
distinguish transformed cells from their normal counterparts. We compared the proteomic
profiles of four glioblastoma multiforme (GBM) tissue samples and four normal brain cortex
samples to examine the molecular basis of gliomagenesis. Trypsin-digested protein samples
were separated by capillary isoelectric focusing with nano-reversed-phase liquid
chromatography and were profiled by mass spectrometric sequencing. Wolf-Hirschhorn syndrome
candidate 1 (WHSC1), along with 103 other proteins, was found only in the GBM proteomes.
Western blot and immunohistochemistry verified our proteomic findings and demonstrated that
30-kDa WHSC1 expression increases with ascending tumor proliferation activity. RNA
interference could suppress glioma cell growth by blocking WHSC1 expression. Our novel
findings encourage the application of proteomic techniques in cancer research.
Key Words:
expression, glioma, proliferation, proteomic profiling, WHSC1
