|
|
|
|
|
|
||
|
|
||||
|
|
||||
|
||||
Molecular Genetics |
Departments of Anatomy (I.F.N., K.M.G., O.B.), Pathology (I.F.N.), and Neurosurgery (O.B.), Medical College of Virginia Campus at Virginia Commonwealth University, Richmond, VA 23298; Department of Neurosurgery, Henry Ford Health Sciences Center, Detroit, MI 48202 (S.A.R.); and GeneSys Technologies, Inc., 1100 Dallas Street, Sauk City, WI 53583 (J.L., J.B.G.)
2 Address correspondence and reprint requests to Oliver Bögler, Ph.D., Departments of Anatomy and Neurosurgery, Virginia Commonwealth University, PO Box 980709, Richmond, VA 23298-0709.
Abstract
The prognosis for most patients with astrocytic glioma is poor, and postoperative life expectancy has not significantly improved in the last decade despite advances in diagnosis, surgery, and adjuvant therapy. Progress has been made, however, in cataloging the genetic alterations that occur in these tumors. Studying the allelic changes using loss of heterozygosity analysis has proven to be a reliable and rapid way of identifying genetic alterations fundamental to the pathology of this disease. In this study, we used a series of fluorescent-labeled markers and a new horizontal ultrathin gel electrophoresis technology (HUGE; GeneSys Technologies, Inc.) to analyze loss of heterozygosity on 11p15 in a series of 24 matched normal/tumor glioma pairs that included both anaplastic astrocytomas and glioblastomas. These studies significantly narrowed the region harboring a putative 11p15.5 glioma-associated gene and further suggest that a second gene involved in the pathogenesis of brain tumors may exist, centromeric, in bands 11p15.5-p15.4.
References
Baffa, R., Negrini, M., Mandes, B., Rugge, M., Ranzini, G.N.,
Hirohaski, S., and Croce, C.M. (1996) Loss of heterozygosity for
chromosome 11 in adenocarcinoma of the stomach. Cancer
Res. 56,268
-272.
Bello, M.J., Vaquero, J., de Campos, J.M., Kusak, M.E., Sarasa, J.L., Saez-Castresana, J., Pestana, A., and Rey, J.A. (1994) Molecular analysis of chromosome 1 abnormalities in human glioma reveals frequent loss of 1p in oligodendroglial tumors. Int. J. Cancer 57,172 -175.[ISI][Medline]
Bögler, O., Huang, H.-J.S., Kleihues, P., and Cavenee, W.K. (1995) The p53 gene and its role in human brain tumors. Glia 15,308 -327.[CrossRef][ISI][Medline]
Cawkwell, L., Bell, S.M., Lewis, F.A., Dixon, M.F., Taylor, G.R., and Quirke, P. (1993) Rapid detection of allele loss in colorectal tumours using microsatellite and fluorescent DNA technology. Br. J. Cancer 67,1262 -1267.[ISI][Medline]
Fults, D., Petronio, J., Noblett, B.D., and Pedone, C.A. (1992) Chromosome 11p15 deletions in human malignant astrocytomas and primitive neuroectodermal tumors. Genomics 14,799 -801.[CrossRef][ISI][Medline]
Furnari, F.B., Huang, H.-J.S., and Cavenee, W.K. (1995) Genetics and malignant progression of human brain tumours. Cancer Surv. 25,233 -275.[ISI][Medline]
Karnik, P., Paris, M., Williams, B.R., Casey, G., Crowe, J., and
Chen, P. (1998) Two distinct tumor suppressor loci within
chromosome 11p15 implicated in breast cancer progression and metastasis.
Hum. Mol. Genet. 7,895
-903.
Kleihues, P., Burger, P.C., and Scheitnauer, B.W. (1993) The new WHO classification of brain tumours. Brain Pathol. 3,255 -268.[ISI][Medline]
Kraus, J.A., Koopmann, J., Kaskel, P., Maintz, D., Brandner, S., Schramm, J., Louis, D.N., Wiestler, O.D., and von Deimling, A. (1995) Shared allelic losses on chromsomes 1p and 19q suggest a common origin of oligodendroglioma and oligoastrocytoma. J. Neuropath. Exp. Neurol. 54,91 -95.[ISI][Medline]
Li, J., Yen, C., Liaw, D., Podsypanina, K., Bose, S., Wang, S.I.,
Puc, J., Miliaresis, C., Rodgers, L., McCombie, R., Bigner, S.H., Giovanella,
B.C., Ittman, M., Tycko, B., Hibshoosh, H., Wigler, M.H., and Parsons, R.
(1997) PTEN, a putative protein tyrosine phosphatase gene mutated
in human brain, breast, and prostate cancer. Science
275,1943
-1947.
Shaw, M., and Knowles, M. (1995) Deletion mapping of chromosome 11 in carcinoma of the bladder. Genes Chromosomes Cancer 13,1 -8.[ISI][Medline]
Sonoda, Y., Lizuka, M., Yasuda, J., Makino, R., Ono, T., Kayama,
T., Yoshimoto, T., and Sekiya, T. (1995) Loss of heterozygosity
at 11p15 in malignant glioma. Cancer Res.
55,2166
-2168.
Steck, P.A., Pershouse, M.A., Jasser, S.A., Yung, W.K.A., Lin, H., Ligon, A.H., Langford, L.A., Baumgard, M.L., Hattier, T., Davis, T., Frye, C., Hu, R., Swedlund, B., Teng, D.H.F., and Tavtigian, S.V. (1997) Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nature Genet. 15,356 -362.[CrossRef][ISI][Medline]
Tran, Y.K., and Newsham, I.F. (1996) High density
marker analysis of 11p15.5 in non-small cell lung carcinomas reveals allelic
deletion of one shared and one distinct region when compared to breast
carcinomas. Cancer Res.
56,2916
-2921.
Tran, Y., Benbatoul, K., Gorse, K., Rempel, S., Futreal, A., Green, M., and Newsham, I. (1998) Novel regions of allelic deletion on chromosome 18p in tumors of the lung, brain and breast. Oncogene 17,3499 -3505.[CrossRef][ISI][Medline]
Viel, A., Giannini, F., Tumiotto, L., Sopracordevole, F., Visentin, M.C., and Boiochhi, M. (1992) Chromosomal localisation of two putative 11p onco-suppressor genes involved in human ovarian tumours. Br. J. Cancer 66,1030 -1036.[Medline]
von Deimling, A., Eibl, R.H., Ohgaki, H., Louis, D.N., von Ammon,
K., Petersen, I., Kleihues, P., Chung, R.Y., Wiestler, O.D., and Seizinger,
B.R. (1992) p53 mutations are associated with 17p allelic loss in
grade II and grade III astrocytoma. Cancer Res.
52,2987
-2990.
Winqvist, R., Hampton, G.M., Mannermaa, A., Blanco, G., Alavaikko,
M., Kiviniemi, H., Taskinen, P.J., Evans, G.A., Wright, F.A., Newsham, I., and
Cavenee, W. (1995) Loss of heterozygosity for chromosome 11 in
primary human breast tumors is associated with poor survival after metastasis.
Cancer Res. 55,2660
-2664.
This article has been cited by other articles:
|
|
 |
|
  D. Mihaila, M. Jankowski, J. A. Gutierrez, M. L. Rosenblum, I. F. Newsham, O. Bogler, and S. A. Rempel Meningiomas: Loss of Heterozygosity on Chromosome 10 and Marker-Specific Correlations with Grade, Recurrence, and Survival Clin. Cancer Res., October 1, 2003; 9(12): 4443 - 4451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Biegel, L. Tan, F. Zhang, L. Wainwright, P. Russo, and L. B. Rorke Alterations of the hSNF5/INI1 Gene in Central Nervous System Atypical Teratoid/Rhabdoid Tumors and Renal and Extrarenal Rhabdoid Tumors Clin. Cancer Res., November 1, 2002; 8(11): 3461 - 3467. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
